Research Methods, Experimental Protocols, and Laboratory Techniques
postdoc wrote:Generally, transfection can be considered as a major laboratory method in integrating protein, RNA, and DNA molecules into tissues and cells. Delivery of molecules encoding genes like plasmid DNA, messenger RNA and small RNA molecules regulating gene expression (microRNA and siRNA)into the primary cells and cancer cell lines have been applied by the scientists.
Transfection efficiencies greatly depends on specific on the type of the cell, reagent used and the protocol applied. But, there is no single transfection reagent that fits different types of cells - scientists have to test multiple reagents and optimize transfection conditions in order to achieve acceptable transfection efficiency withouit inducing cellular cytotoxicity. There are several pre-optimized transfection reagents commercially available from Altogen Biosystems (http://www.altogen.com) for such cell lines as: MEF, A549, HepG2, Fibroblast, VERO, and many others. Also, in vivo transfection kits allow delivery of protein, DNA, and RNA molecules into animals (http://www.altogen.com/mirna.php).
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