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What is Bioremediation and Oil-degrading microorganisms?

PostPosted: Mon Sep 28, 2009 2:57 pm
by biologist
What is Bioremediation and Oil-degrading microorganisms?

Re: What is Bioremediation and Oil-degrading microorganisms?

PostPosted: Fri Apr 22, 2011 7:46 pm
by ATGC2001
Microbial degradation is natural mechanism to cleanup the petroleum hydrocarbon pollutants (and crude oil) from the environment. The recognition of biodegraded petroleum-derived aromatic hydrocarbons in marine sediments was reported by Jones et al - demonstrating that multiple microorganisms are capable to degrade crude oil, including Arthrobacter, Burkholderia, Mycobacterium, Pseudomonas, Sphingomonas, and Rhodococcus.

An important requirement for successful bioremediation is the presence of microorganisms with appropriate metabolic capabilities dependent on multiple factores such as nutrients, oxygen, and pH. There are the two main approaches to oil spill bioremediation: (a) bioaugmentation, in which known oil-degrading bacteria are added to supplement the existing microbial population, and (b) biostimulation, in which the growth of indigenous oil degraders is stimulated by the addition of nutrients or other growth-limiting cosubstrates.

Biodegradation of petroleum hydrocarbons is a complex process that depends on the nature and on the amount of the hydrocarbons present. Petroleum hydrocarbons can be divided into four classes: the saturates, the aromatics, the asphaltenes (phenols, fatty acids, ketones, esters, and porphyrins), and the resins (pyridines, quinolines, carbazoles, sulfoxides, and amides).

Re: What is Bioremediation and Oil-degrading microorganisms?

PostPosted: Fri Apr 22, 2011 7:51 pm
by transfection
Texas-Based Biotechnology Company Develops Oil-Degrading Bacteria

Altogen Labs ( announced that it has successfully isolated natural oil-eating bacteria from polluted soil near Galveston Bay, Texas that has been shown in laboratory tests to be effective for the bioremediation of crude oil and petroleum saturated earth.

"We started this project 6 months ago by taking soil in a heavily oil-contaminated area (near Galveston Bay, TX) and have isolated strains of naturally present bacteria. We are currently finishing the proof-of-concept experiments demonstrating that these strains are effectively degrading Texas oil. Methods based on bioremediation approach are actively used in several countries now and well accepted by nature while posing minimal threat to marine ecosystems," said Dr.Dmitriy Ovcharenko, Altogen Labs CEO.

Bioremediation is a process involving microorganisms, fungi, green plants, or their enzymes to restore the natural environment to its original state after contamination. Bioremediation of the environment, be it soil or water, following contamination by crude oil, is performed by a variety of bacteria that feed on hydrocarbons, breaking down the petroleum molecules into less harmful substances. These bacteria, which occur naturally in the environment, can be isolated and expanded to help remediate oil spills. According to the Environmental Protection Agency, applied remediation of oil contaminated soil and water is a high priority of government and industry as this method can be quicker and cheaper - today's technology for cleaning up oil spills relies on physical cleaning methods, including skimmers, vacuums, and in situ burning, with chemical cleaning methods often toxic and poisonous to marine life. The recent spill of millions of gallons of oil in the Gulf of Mexico demonstrated a lack of effective technologies to clean up oil from the ocean as well as from large areas of contaminated soil.

Altogen Labs scientists have found these oil-degrading bacterial strains to be naturally present in various types of soil and water; however, upon contamination with crude oil, the concentration of bacteria increases dramatically because these bacteria feed on oil. Unfortunately, this process only occurs on the edge of the oil spill, as most microorganisms need other nutrients and oxygen to survive, making the ecosystem relatively inefficient for bioremediation. Altogen Labs ( developed a technology that allows the acceleration of this natural process. Scientists were able to identify a hydrocarbon-degrading bacterial population, including saturate degraders and polycyclic aromatic hydrocarbon degraders, and optimize the growing process in the laboratory. They developed a method of cultivation of oil-degrading bacteria that are specific to the site of a particular spill - microbes that act on the hydrocarbon molecules that are present at the site of the pollution.

The remarkable feature of this development is that bacteria can be expanded in large aqueous volumes and then the water can be evaporated to store high concentrations of bacteria in dry form and low volume. The product is very stable, and dry, natural bacteria can be activated by the addition of water. The growth conditions of these natural bacteria were optimized for effective oil degradation in Texas soil (including variations in temperature and soil conditions, as well as a number of oil compositions).

Altogen Labs is actively working on further development of this technology for both soil and water applications. However, the current product development process is limited to laboratory scale, and industrial-grade production and commercial product launch will require additional scale-up work. The company is now looking for collaborative opportunities to enable this large-scale development project.


Re: What is Bioremediation and Oil-degrading microorganisms?

PostPosted: Sun Nov 27, 2011 2:10 pm
by transfection
Bioremediation in Texas: Oil-degrading bacteria products and services:

Re: What is Bioremediation and Oil-degrading microorganisms?

PostPosted: Tue Oct 30, 2012 10:41 am
by biologist
Altogen Labs Announces Patent Filing for Bioremediation Process and Oil Spill Cleanup Products

Altogen Labs Announces Patent Filing for Bioremediation Process and Oil Spill Cleanup Products

Altogen Labs announced the filing of a patent for bioremediation microorganism isolation and selection methods (USPTO application 13/490,508). This environmental technology is based on isolation of naturally present microorganisms in heavily contaminated soil, which allows accelerating natural bioremediation processes. We don’t have to wait hundreds of years for nature to degrade an oil spill or clean up land contaminated with toxic chemicals and carcinogens. Instead, by reintroducing natural bacteria to oil spill sites, Altogen Labs accomplish oil degradation process in just a few months. This bioaugmentation platform technology to remediate crude oil and chemical spills can be applied worldwide.

Both laboratory and field tests showed that Altogen Labs site-specific bacterial strains are highly effective for remediation of crude oil- and petroleum-saturated earth. The bacteria is grown in the lab to speed up the natural process and then re-introduced to the contaminated site. Multiple lab studies show that after consuming the oil and having no remaining food source, the bacteria die. No adverse effects of using the live bacteria have been observed, and the effectiveness and safety of the method are remarkable. Scientists at Altogen Labs used functional genomics to perform whole genome sequencing study to characterize these newly identified natural microorganisms and identify specific biosurfactant and quorum-sensing genes that allow the microorganisms to work together for efficient and effective crude oil spill bioremediation.

Altogen Labs also offers soil remediation services and development programs focused on other chemical contaminations besides crude oil, including remediation of soil with recalcitrant organic chemical spills including polycyclic aromatic hydrocarbons, petroleum, pesticides, herbicides, perchlorate, carbon tetrachloride, chloroform, acetone, benzene, toluene, xylenes, perchloroethylene, chlorofluorocarbon, aliphatic chlorinated hydrocarbons, and other industrial and chemical manufacturing wastes and spills.

Altogen Labs announced that site-specific bioremediation products will be manufactured and sold through its exclusive distributor - Soil Bioremediation Inc ( located in Austin, Texas.

About Altogen Labs

Altogen Labs is GLP compliant laboratory that provides biotechnology research services and biology CRO services to pharmaceutical, biotechnology, and academic institutions worldwide. Bioremediation is one of the company’s focus areas. This bioaugmentation-based soil remediation technology provides conversion of toxic, environment-contaminating compounds into innocuous substances by using natural oil-degrading bacteria.

Altogen Labs
4020 South Industrial Dr, Suite 130
Austin TX 78744 USA
(512) 433-6177